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dc.contributor.authorKoeduka, Takao
dc.contributor.authorIshizaki, Kimitsune
dc.contributor.authorMwenda, Cynthia Mugo
dc.contributor.authorHori, Koichi
dc.contributor.authorSasaki-Sekimoto, Yuko
dc.contributor.authorOhta, Hiroyuki
dc.contributor.authorKohchi, Takayuki
dc.contributor.authorMatsui, Kenji
dc.date.accessioned2021-10-25T08:05:37Z
dc.date.available2021-10-25T08:05:37Z
dc.date.issued2015
dc.identifier.citationKoeduka, T., Ishizaki, K., Mwenda, C. M., Hori, K., Sasaki-Sekimoto, Y., Ohta, H., Kohchi, T., & Matsui, K. (2015). Biochemical characterization of allene oxide synthases from the liverwort Marchantia polymorpha and green microalgae Klebsormidium flaccidum provides insight into the evolutionary divergence of the plant CYP74 family. Planta, 242(5), 1175–1186. https://doi.org/10.1007/s00425-015-2355-8en_US
dc.identifier.urihttp://repository.must.ac.ke/handle/123456789/413
dc.description.abstractAllene oxide synthase (AOS) and hydroperoxide lyase (HPL) mediate the formation of precursors of jasmonates and carbon-six volatiles, respectively. AOS and HPL utilize fatty acid hydroperoxides and belong to the plant cytochrome P450 74 (CYP74) family that mediates plant defense against herbivores, pathogens, or abiotic stresses. Although members of the CYP74 family have been reported in mosses and other species, the evolution and function of multiple CYP74 genes in plants remain elusive. Here, we show that the liverwort Marchantia polymorpha belongs to a basal group in the evolution of land plants; has two closely related proteins (59 % identity), MpAOS1 and MpAOS2, that are similar to moss PpAOS1 (49 and 47 % identity, respectively); and exhibits AOS activity but not HPL activity. We also found that the green microalgae Klebsormidium flaccidum, consist of multicellular and non-branching filaments, contains an enzyme, KfAOS, that is similar to PpAOS1 (37 % identity), and converts 13-hydroperoxide of linolenic acid to 12-oxo-phytodienoic acid in a coupled reaction with allene oxide cyclase. Phylogenetic analysis showed two evolutionarily distinct clusters. One cluster comprised AOS and HPL from charophytic algae, liverworts, and mosses, including MpAOSs and KfAOS. The other cluster was formed by angiosperm CYP74. Our results suggest that plant CYP74 enzymes with AOS, HPL, and divinyl ether synthase activities have arisen multiple times and in the two different clades, which occurred prior to the divergence of the flowering plant lineage.en_US
dc.language.isoenen_US
dc.publisherSpringer Berlin Heidelbergen_US
dc.subjectAllene Oxide Synthaseen_US
dc.subjectPlant Evolutionen_US
dc.subjectCYP74en_US
dc.subjectHydroperoxide Lyaseen_US
dc.subjectC8 Volatilesen_US
dc.titleBiochemical Characterization of Allene Oxide Synthases from the Liverwort Marchantia Polymorpha and Green Microalgae Klebsormidium Flaccidum Provides Insight into the Evolutionary Divergence of the Plant CYP74 Familyen_US
dc.typeArticleen_US


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