The linseed plant produces linseed, an excellent source of health-promoting omega-3 fatty acid, alpha linolenic acid. Currently, there is negligible production of this crop in Kenya. This study aimed to determine the growth and yield potential of five linseed varieties at different nitrogen fertilizer levels in Juja, Central Kenya. Methodology and results: Five linseed varieties namely Summit, S19/21, Raja, Jawhar and S19/12 were evaluated at 0, 75 and 150KgN/ha fertilizer levels in Juja area, Kenya. Effect of the fertilizer on number of leaves, height, tillering, dry weight, number of heads and seed yield were evaluated in two planting seasons. Numbers of leaves, height, tillering and dry weight were not significantly affected by nitrogen application (P>0.05). Raja yielded higher head and seed yield than the other varieties in both seasons (P<0.05). The highest head yield was obtained at 75KgN/ha during the February- June season while it was 150KgN/ha during the July-December season. Conclusion: Nitrogen fertilization up to 150KgN/ha does not result in significant differences in growth of Summit, S19/21, Raja, Jawhar and S19/12 and can so be considered a moderate consumer of nitrogen. Raja yields more highly compared to the other four varieties. Hence, it can be recommended for adoption in the study area as well as for future research and breeding programs.