| dc.contributor.author | Ouma, LA | |
| dc.contributor.author | Owino, VA | |
| dc.contributor.author | Gichuki, CW | |
| dc.contributor.author | Masiga, DK | |
| dc.date.accessioned | 2018-09-05T08:05:28Z | |
| dc.date.accessioned | 2020-02-07T05:25:17Z | |
| dc.date.available | 2018-09-05T08:05:28Z | |
| dc.date.available | 2020-02-07T05:25:17Z | |
| dc.date.issued | 2014 | |
| dc.identifier.uri | http://repository.must.ac.ke/handle/123456789/1131 | |
| dc.description.abstract | In the course of a Human African Trypanosomiasis (HAT) infection, there is a protracted and fluctuating course of parasitaemia during which different variant surface glycoproteins (VSGs) are expressed in a hierarchical fashion, where some VSGs appear preferentially early in infection and others only later. VSG 4; previously found to be one of the most frequently expressed VSGs in early stage of T. b. rhodesiense infections, was chosen as a putative diagnostic VSG candidate in this study. A total of 67 human blood samples from Kenya and Uganda were tested using a VSG 4 based Polymerase Chain Reaction (PCR). VSG 4 protein was also expressed in E. coli and tested for reactivity with HAT patient sera. A detection of 95.8% and 86% among blood samples from Kenya and Uganda respectively was observed in this PCR. Western blot showed a smear around 55-65KDa; probably a VSG, since VSGs have molecular weights in this region. These results suggest that the detection of VSG 4 would make a more sensitive diagnostic assay in the early diagnosis and treatment follow-up of T. b. rhodesiense infections. | en_US |
| dc.language.iso | en | en_US |
| dc.publisher | Integrated Publishing Association | en_US |
| dc.subject | Variant surface glycoprotein (VSG), Trypanosoma brucei rhodesiense, East African sleeping sickness, Diagnostic candidate. | en_US |
| dc.title | Variant Surface Glycoprotein 4, a potential diagnostic candidate for the detection of Trypanosoma brucei rhodesiense infections | en_US |
| dc.type | Article | en_US |
