• Login
    View Item 
    •   Repository Home
    • Staff Publications
    • School of Agriculture & Food Science
    • View Item
    •   Repository Home
    • Staff Publications
    • School of Agriculture & Food Science
    • View Item
    JavaScript is disabled for your browser. Some features of this site may not work without it.

    Cost-effective nutrient sources for tissue culture of cassava (Manihot esculenta Crantz)

    Thumbnail
    View/Open
    Cost-effectivenutrientsourcesfortissuecultureofcassavaManihotesculentaCrantz.pdf (535.4Kb)
    Date
    2012
    Author
    Ogero, KO
    Gitonga, NM
    Ombori, O
    Ngugi, M
    Metadata
    Show full item record
    Abstract
    Application of tissue culture technology is constrained by high costs making seedlings unaffordable. The objective of this study was to evaluate the possibility of using locally available fertilizers as alternative nutrient sources for cassava micropropagation. A Low Cost Medium (LCM) whereby the conventional sources of four Murashige and Skoog ( MS) macronutrients had been replaced with locally available fertilizers was developed. Stanes Iodized Microfood from Osho Chemical Industries in Nairobi was used as the alternative source of micronutrients. Modified conventional MS medium was used as the control. Both media were supplemented with 30 g/l of table sugar and 3 g/l of gelrite. Two cassava varieties, Muchericheri and KME 1 were regenerated on the two media. Node, leaf and root formation patterns plus plant height were determined and compared.A reduction of 95.50% in nutrient cost was achieved. The two cassava varieties had a significantly (p<0.05) higher number of nodes on the conventional medium compared to LCM. There were no differences in node formation by the two varieties on the low cost medium during both initiation and multiplication. KME 1 produced significantly more leaves on the LCM compared to Muchericheri during both initiation and multiplication. Acclimatization of plantlets was successful;hence,this protocol can be adopted in cassava regeneration.
    URI
    http://repository.must.ac.ke/handle/123456789/1142
    Collections
    • School of Agriculture & Food Science [251]

    MUST Repository copyright © 2002-2016  MUST Repository
    Contact Us | Send Feedback
    Theme by 
    MUST Repository
     

     

    Browse

    All of the RepositoryCommunities & CollectionsBy Issue DateAuthorsTitlesSubjectsThis CollectionBy Issue DateAuthorsTitlesSubjects

    My Account

    LoginRegister

    MUST Repository copyright © 2002-2016  MUST Repository
    Contact Us | Send Feedback
    Theme by 
    MUST Repository