| dc.contributor.author | Awuoche, Erick Otieno | |
| dc.contributor.author | Weiss, Brian L | |
| dc.contributor.author | Mireji, Paul O | |
| dc.contributor.author | Vigneron, Aurélien | |
| dc.contributor.author | Nyambega, Benson | |
| dc.contributor.author | Murilla, Grace | |
| dc.contributor.author | Aksoy, Serap | |
| dc.date.accessioned | 2018-12-13T07:51:03Z | |
| dc.date.accessioned | 2020-02-07T06:08:28Z | |
| dc.date.available | 2018-12-13T07:51:03Z | |
| dc.date.available | 2020-02-07T06:08:28Z | |
| dc.date.issued | 2018-12 | |
| dc.identifier.citation | Awuoche EO, Weiss BL, Mireji PO, Vigneron A, Nyambega B, Murilla G, et al., Expression profiling of Trypanosoma congolense genes during development in the tsetse fly vector Glossina morsitans morsitans. Parasit Vectors. 2018; 11(1):380. Epub 2018/07/05. https://doi.org/10.1186/s13071-018- 2964-8 | en_US |
| dc.identifier.uri | https://doi.org/10.1186/s13071-018-2964-8 | |
| dc.identifier.uri | http://repository.must.ac.ke/handle/123456789/1164 | |
| dc.description.abstract | The tsetse transmitted parasitic flagellate Trypanosoma congolense causes animal African trypanosomosis (AAT) across sub-Saharan Africa. AAT negatively impacts agricultural, economic, nutritional and subsequently, health status of the affected populace. The molecular mechanisms that underlie T. congolense’s developmental program within tsetse are largely unknown due to considerable challenges with obtaining sufficient parasite cells to perform molecular studies. In this study, we used RNA-seq to profile T. congolense gene expression during development in two distinct tsetse tissues, the cardia and proboscis. Indirect immunofluorescent antibody test (IFA) and confocal laser scanning microscope was used to localize the expression of a putative protein encoded by the hypothetical protein (TcIL3000_0_02370). Consistent with current knowledge, genes coding several variant surface glycoproteins (including metacyclic specific VSGs), and the surface coat protein, congolense epimastigote specific protein, were upregulated in parasites in the proboscis (PB-parasites). Additionally, our results indicate that parasites in tsetse’s cardia (C-parasites) and PB employ oxidative phosphorylation and amino acid metabolism for energy. Several genes upregulated in C-parasites encoded receptor-type adenylate cyclases, surface carboxylate transporter family proteins (or PADs), transport proteins, RNA-binding proteins and procyclin isoforms. Gene ontology analysis of products of genes upregulated in C-parasites showed enrichment of terms broadly associated with nucleotides, microtubules, cell membrane and its components, cell signaling … | en_US |
| dc.language.iso | en | en_US |
| dc.publisher | BioMed Central | en_US |
| dc.subject | Confocal microscopy | en_US |
| dc.subject | Gene expression analysis | en_US |
| dc.subject | Glossina morsitans morsitans | en_US |
| dc.subject | Tsetse cardia | en_US |
| dc.subject | Trypanosoma congolense | en_US |
| dc.subject | Tsetse proboscis | en_US |
| dc.title | Expression profiling of Trypanosoma congolense genes during development in the tsetse fly vector Glossina morsitans morsitans | en_US |
| dc.type | Article | en_US |
