Over the last few decades there has been a paradigm shift from teacher-centered to student-centered learning. The teachers have been more of facilitator ensuring learning objectives are met. Problem Based Learning (PBL) is considered one of the most favorable method in Student-Centered learning. From this point of view Moi University adopted the PBL. This study aims to assess the lecturers’ knowledge and perception regarding problem-based learning (PBL). Participants were 65 lecturers in the College of Health Sciences. Self-administered questionnaires were used in data collection. Data analysis done utilizing SPSS 22. The overall response rate was 100 % (N=65), 38% (25) had PBL knowledge at joining program, 34 (52%) viewed program graduates as independent, innovative and creative. While on SPICES model 19 (29%) believe made students lifelong learners and 12 (19%) creates team-work spirit among the students and 75% of the lecturers anticipated to be trained. Also results indicated, lecturers did not prefer mixed method of teaching compared to problem-based learning (PBL) not statistically significant(p<0.05). A medium proportion of lecturers had good knowledge of PBL a high number with favorable attitudes towards PBL. Training courses by the college should be considered for the staff that had not previously been trained using PBL.