| dc.identifier.citation | Zuo, C., Hu, Q., Su, A., Pei, F., Ma, G., Xu, H., Xie, M., Liu, J., Mariga, A. M., & Yang, W. (2021). Transcriptome analysis reveals the underlying mechanism of nanocomposite packaging in delaying quality deterioration of Flammulina velutipes. Postharvest Biology and Technology, 182, 111723. https://doi.org/10.1016/j.postharvbio.2021.111723 | en_US |
| dc.description.abstract | To expound the molecular mechanism of nanocomposite packaging material (Nano-PM) in delaying quality deterioration of Flammulina velutipes during storage, transcriptome analysis was conducted to observe the changes of gene expression in F. velutipes that was packaged in Nano-PM, polyethylene packaging material (Normal-PM) and no packaging material (No-PM). In comparison with Normal-PM, 379 differentially expressed genes (DEGs) were identified in Nano-PM packaged F. velutipes, comprising of 161 up-regulated genes and 218 down-regulated genes. Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analysis indicated that Nano-PM improved the stress resistance of F. velutipes by up-regulating RBOHF and msp2, which were related to reactive oxygen species (ROS) metabolism. Nano-PM also ensured sufficient energy supply by regulating oxidative phosphorylation and glycolysis/gluconeogenesis. Moreover, the regulation of gene expression also delayed programmed cell death (PCD) and lignification. Furthermore, the quantitative real-time PCR (qRT-PCR) results were also in accordance with transcriptome analysis. Therefore, Nano-PM could regulate the expression of specific genes, which in turn alleviated quality deterioration of F. velutipes during storage. | en_US |
