• Login
    View Item 
    •   Repository Home
    • Staff Publications
    • School of Pure and Applied Sciences
    • View Item
    •   Repository Home
    • Staff Publications
    • School of Pure and Applied Sciences
    • View Item
    JavaScript is disabled for your browser. Some features of this site may not work without it.

    Biochemical Characterization of Allene Oxide Synthases from the Liverwort Marchantia Polymorpha and Green Microalgae Klebsormidium Flaccidum Provides Insight into the Evolutionary Divergence of the Plant CYP74 Family

    Thumbnail
    View/Open
    Abstract (6.257Kb)
    Date
    2015
    Author
    Koeduka, Takao
    Ishizaki, Kimitsune
    Mwenda, Cynthia Mugo
    Hori, Koichi
    Sasaki-Sekimoto, Yuko
    Ohta, Hiroyuki
    Kohchi, Takayuki
    Matsui, Kenji
    Metadata
    Show full item record
    Abstract
    Allene oxide synthase (AOS) and hydroperoxide lyase (HPL) mediate the formation of precursors of jasmonates and carbon-six volatiles, respectively. AOS and HPL utilize fatty acid hydroperoxides and belong to the plant cytochrome P450 74 (CYP74) family that mediates plant defense against herbivores, pathogens, or abiotic stresses. Although members of the CYP74 family have been reported in mosses and other species, the evolution and function of multiple CYP74 genes in plants remain elusive. Here, we show that the liverwort Marchantia polymorpha belongs to a basal group in the evolution of land plants; has two closely related proteins (59 % identity), MpAOS1 and MpAOS2, that are similar to moss PpAOS1 (49 and 47 % identity, respectively); and exhibits AOS activity but not HPL activity. We also found that the green microalgae Klebsormidium flaccidum, consist of multicellular and non-branching filaments, contains an enzyme, KfAOS, that is similar to PpAOS1 (37 % identity), and converts 13-hydroperoxide of linolenic acid to 12-oxo-phytodienoic acid in a coupled reaction with allene oxide cyclase. Phylogenetic analysis showed two evolutionarily distinct clusters. One cluster comprised AOS and HPL from charophytic algae, liverworts, and mosses, including MpAOSs and KfAOS. The other cluster was formed by angiosperm CYP74. Our results suggest that plant CYP74 enzymes with AOS, HPL, and divinyl ether synthase activities have arisen multiple times and in the two different clades, which occurred prior to the divergence of the flowering plant lineage.
    URI
    http://repository.must.ac.ke/handle/123456789/413
    Collections
    • School of Pure and Applied Sciences [170]

    MUST Repository copyright © 2002-2016  MUST Repository
    Contact Us | Send Feedback
    Theme by 
    MUST Repository
     

     

    Browse

    All of the RepositoryCommunities & CollectionsBy Issue DateAuthorsTitlesSubjectsThis CollectionBy Issue DateAuthorsTitlesSubjects

    My Account

    LoginRegister

    MUST Repository copyright © 2002-2016  MUST Repository
    Contact Us | Send Feedback
    Theme by 
    MUST Repository