| dc.contributor.author | Nyamota, Richard | |
| dc.contributor.author | Owino, Vincent | |
| dc.contributor.author | Murungi, Edwin Kimathi | |
| dc.contributor.author | Villinger, Jandouwe | |
| dc.contributor.author | Otiende, Moses | |
| dc.contributor.author | Masiga, Daniel | |
| dc.contributor.author | Thuita, John | |
| dc.contributor.author | Lekolool, Isaac | |
| dc.contributor.author | Jeneby, Maamun | |
| dc.date.accessioned | 2021-12-06T12:44:05Z | |
| dc.date.available | 2021-12-06T12:44:05Z | |
| dc.date.issued | 2020 | |
| dc.identifier.uri | http://repository.must.ac.ke/handle/123456789/437 | |
| dc.description.abstract | Background: Simian immunodeficiency virus (SIV) naturally infects African non-human primates (NHPs) and poses a threat of transmission to humans through hunting and consumption of monkeys as bushmeat. This study investigated the as of yet unknown molecular diversity of SIV in free-ranging Chlorocebus species (African green monkeys-AGMs) and Papio anubis (olive baboons) within Mombasa, Kisumu and Naivasha urban centres in Kenya.
Methods: We collected blood samples from 124 AGMs and 65 olive baboons in situ, and detected SIV by high-resolution melting analysis and sequencing of PCR products.
Results: Simian immunodeficiency virus prevalence was 32% in AGMs and 3% in baboons. High-resolution melting (HRM) analysis demonstrated distinct melt profiles illustrating virus diversity confirmed by phylogenetic analysis.
Conclusions: There is persistent evolutionary diversification of SIVagm strains in its natural host, AGMs and cross-species infection to olive baboons is occurring. Further study is required to establish pathogenesis of the diverse SIVagm variants and baboon immunological responses. | en_US |
| dc.language.iso | en | en_US |
| dc.publisher | Wiley | en_US |
| dc.subject | African green monkeys | en_US |
| dc.subject | SIVagm | en_US |
| dc.subject | Evolution | en_US |
| dc.subject | High-resolution Melting Analysis | en_US |
| dc.subject | Olive baboon | en_US |
| dc.subject | Strain Diversity. | en_US |
| dc.title | Broad diversity of simian immunodeficiency virus infecting Chlorocebus species (African green monkey) and evidence of cross-species infection in Papio anubis (olive baboon) in Kenya | en_US |
| dc.type | Article | en_US |
