| dc.description.abstract | The hydroperoxide lyase (HPL) pathway for six carbon (C6) volatiles and the allene oxide synthase (AOS) pathway for
jasmonates (JAs) share the first part of the pathway. To avoid competition, a separate localization of HPL and AOS
might be important. A fusion protein comprising Arabidopsis HPL and green fluorescent protein was transported into
chloroplasts, where AOS was located. Arabidopsis harboring β-glucuronidase (GUS) gene downstream of Arabidopsis
HPL promoter (pAtHPL::GUS) showed different GUS activity in floral organs compared with that from pAtAOS::GUS.
With pAtHPL::GUS, wounding enhanced GUS activity at the periphery of cotyledons; while with pAtAOS::GUS, GUS
activity was high in the vasculature. The distribution of the ability to form C6 volatiles correlated with the profile of
HPL promoter activity; however, this ability unchanged after wounding. Inconsistency between the AOS promoter
activity and JA levels was also evident. Thus, an additional factor should also control the ability to form C6 volatiles
and JAs. | en_US |
