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dc.contributor.authorMworia, E. G.
dc.contributor.authorYoshikawa, T.
dc.contributor.authorYokotani, N
dc.contributor.authorFukuda, T.
dc.contributor.authorSuezawa, K.
dc.contributor.authorUshijima, K.
dc.contributor.authorNakano, R.
dc.contributor.authorKubo, Y.
dc.date.accessioned2021-12-16T09:52:05Z
dc.date.available2021-12-16T09:52:05Z
dc.date.issued2010
dc.identifier.citationMworia, Eric & Yoshikawa, Takashi & Yokotani, Naoki & Fukuda, Tetsuo & Suezawa, Katsuhiko & Ushijima, Koichiro & Nakano, Ryohei & Kubo, Yasutaka. (2010). Characterization of ethylene biosynthesis and its regulation during fruit ripening in kiwifruit, Actinidia chinensis ‘Sanuki Gold’. Postharvest Biology and Technology. 55. 108-113. 10.1016/j.postharvbio.2009.08.007.en_US
dc.identifier.issn0925-5214
dc.identifier.urihttps://www.sciencedirect.com/science/article/pii/S0925521409001781
dc.identifier.urihttp://repository.must.ac.ke/handle/123456789/635
dc.description.abstractEthylene biosynthesis in kiwifruit, Actinidia chinensis ‘Sanuki Gold’ was characterized using propylene, an ethylene analog, and 1-methylcyclopropene (1-MCP), an inhibitor of ethylene perception. In fruit harvested between a young stage (66 days after pollination) (DAP) and an early commercial harvesting stage (143 DAP), 2 days of exposure to propylene were sufficient to initiate ethylene biosynthesis while in fruit harvested at commercial harvesting stage (154 DAP), 4 days of propylene treatment were required. This observation suggests that response of ethylene biosynthesis to propylene treatment in kiwifruit declined with fruit maturity. Propylene treatment resulted in up-regulated expression of AC-ACO1, AC-ACO2, AC-SAM1 and AC-SAM2, prior to the induction of AC-ACS1 and ethylene production, confirming that AC-ACS1 is the rate limiting step in ethylene biosynthesis in kiwifruit. Treatment of fruit with more than 5μLL−1 of 1-MCP after the induction of ethylene production subsequently suppressed ethylene production and expression of ethylene biosynthesis genes. Treatment of fruit with 1-MCP at harvest followed with propylene treatment delayed the induction of ethylene production and AC-ACS1 expression for 5 days. These observations suggest that in ripening kiwifruit, ethylene biosynthesis is regulated by positive feedback mechanism and that 1-MCP treatment at harvest effectively delays ethylene production by 5 days.en_US
dc.language.isoenen_US
dc.publisherElsevieren_US
dc.subjectAutocatalyticen_US
dc.subjectEthylene biosynthesisen_US
dc.subjectKiwifruiten_US
dc.subject1-Methylcyclopropeneen_US
dc.titleCharacterization of ethylene biosynthesis and its regulation during fruit ripening in kiwifruit, Actinidia chinensis ‘Sanuki Gold’en_US
dc.typeArticleen_US


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