dc.contributor.author | Mworia, E. G. | |
dc.contributor.author | Yoshikawa, T. | |
dc.contributor.author | Yokotani, N | |
dc.contributor.author | Fukuda, T. | |
dc.contributor.author | Suezawa, K. | |
dc.contributor.author | Ushijima, K. | |
dc.contributor.author | Nakano, R. | |
dc.contributor.author | Kubo, Y. | |
dc.date.accessioned | 2021-12-16T09:52:05Z | |
dc.date.available | 2021-12-16T09:52:05Z | |
dc.date.issued | 2010 | |
dc.identifier.citation | Mworia, Eric & Yoshikawa, Takashi & Yokotani, Naoki & Fukuda, Tetsuo & Suezawa, Katsuhiko & Ushijima, Koichiro & Nakano, Ryohei & Kubo, Yasutaka. (2010). Characterization of ethylene biosynthesis and its regulation during fruit ripening in kiwifruit, Actinidia chinensis ‘Sanuki Gold’. Postharvest Biology and Technology. 55. 108-113. 10.1016/j.postharvbio.2009.08.007. | en_US |
dc.identifier.issn | 0925-5214 | |
dc.identifier.uri | https://www.sciencedirect.com/science/article/pii/S0925521409001781 | |
dc.identifier.uri | http://repository.must.ac.ke/handle/123456789/635 | |
dc.description.abstract | Ethylene biosynthesis in kiwifruit, Actinidia chinensis ‘Sanuki Gold’ was characterized using propylene, an ethylene analog, and 1-methylcyclopropene (1-MCP), an inhibitor of ethylene perception. In fruit harvested between a young stage (66 days after pollination) (DAP) and an early commercial harvesting stage (143 DAP), 2 days of exposure to propylene were sufficient to initiate ethylene biosynthesis while in fruit harvested at commercial harvesting stage (154 DAP), 4 days of propylene treatment were required. This observation suggests that response of ethylene biosynthesis to propylene treatment in kiwifruit declined with fruit maturity. Propylene treatment resulted in up-regulated expression of AC-ACO1, AC-ACO2, AC-SAM1 and AC-SAM2, prior to the induction of AC-ACS1 and ethylene production, confirming that AC-ACS1 is the rate limiting step in ethylene biosynthesis in kiwifruit. Treatment of fruit with more than 5μLL−1 of 1-MCP after the induction of ethylene production subsequently suppressed ethylene production and expression of ethylene biosynthesis genes. Treatment of fruit with 1-MCP at harvest followed with propylene treatment delayed the induction of ethylene production and AC-ACS1 expression for 5 days. These observations suggest that in ripening kiwifruit, ethylene biosynthesis is regulated by positive feedback mechanism and that 1-MCP treatment at harvest effectively delays ethylene production by 5 days. | en_US |
dc.language.iso | en | en_US |
dc.publisher | Elsevier | en_US |
dc.subject | Autocatalytic | en_US |
dc.subject | Ethylene biosynthesis | en_US |
dc.subject | Kiwifruit | en_US |
dc.subject | 1-Methylcyclopropene | en_US |
dc.title | Characterization of ethylene biosynthesis and its regulation during fruit ripening in kiwifruit, Actinidia chinensis ‘Sanuki Gold’ | en_US |
dc.type | Article | en_US |