Isolation, characterization and HepG-2 inhibition of a novel proteoglycan from Flammulina velutipes.
View/ Open
Date
2021Author
Xu, Hui
Hu, Ye
Hu, Qiuhui
Liu, Jianhui
Su, Anxiang
Xie, Minhao
Ma, Gaoxing
Pei, Fei
Mariga, Alfred Mugambi
Yang, Wenjian
Metadata
Show full item recordAbstract
Flammulina velutipes has anti-inflammatory, immunomodulatory, antioxidant and many bioactive properties with high contents of carbohydrate, proteins and fibers. In this study, a novel proteoglycan with polysaccharide complexes and protein chain, named PGD1-1, was isolated from F. velutipes. The structural characteristics of PGD1-1 were then determined, and its anti-proliferation and pro-apoptotic activities against HepG-2 cells were demonstrated in vitro. Results proved that the average molecular weight of PGD1-1 was 32.71 kDa, and the carbohydrate and protein contents were 93.35 and 2.33%, respectively. The protein moiety was bonded to a polysaccharide chain via O-glycosidic linkage. The monosaccharides consisted of d-glucose, D-galactose and D-xylose in a molar ratio of 21.90:2.84:1.00. PGD1-1 significantly inhibited the proliferation of HepG-2 cells by affecting cell lipid peroxidation and nitric oxide production. In addition, PGD1-1 promoted the apoptosis of HepG-2 cells, especially the early apoptosis. These findings proved that PGD1-1 was a novel potent ingredient against the proliferation of HepG-2, which will provide a theoretical basis for the development and utilization of the functional ingredients of the F. velutipes.
URI
https://doi.org/10.1016/j.ijbiomac.2021.08.086http://repository.must.ac.ke/handle/123456789/378